Coding

Part:BBa_M31520:Design

Designed by: Han Zhu   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-27)


g8 modified to be independent of g3 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 229
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 229
    Illegal BglII site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 229
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 229
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All point mutations made were checked to make sure they really are silent mutations that will not change the amino acid sequence of p8.


Source

atgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattTacTtcCaaGgcGagTtgaGAATTC

References